Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film. Aliquoting of 10x buffer is recommended if many small experiments are to be performed. 2. Thaw 10x buffer at 24-30°C, mixing end-over-end. 3. Dilute 10X Cell Lysis Buffer to a 1X solution using ddH2O. This product supplies enough 10X material to make 150mls of whole cell extract. 4. Chill 1X buffer on ice and add PMSF just prior to use. The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3). Immunohistochemistry (IHC) staining allows for 2 broad classes of detection: 1) chromogenic and 2) fluorescent. For chromogenic detection, CST recommends using polymer-based systems that avoid the limitations of the biotin-based system while also increasing sensitivity of the assay. Try SignalStain ® Boost IHC Detection Reagent (HRP, …Monoclonal Antibody for studying LC3B. Cited in 138 publications. Validated for WB, IP, IHC, IF, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, LC3B (E5Q2K) Mouse Monoclonal Antibody (CST #83506) is ready to ship.Lyse cells by adding 1X SDS sample buffer (100 µl per well of 6-well plate or 500 µl for a 10 cm diameter plate). Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Checking the Cell Signaling Technology ® (CST ®) website, you are in luck! There are mouse reactive flow-validated antibody conjugates available against each target: CD4 and TCRβ . While you know that each protein is found at the plasma membrane, you need to check whether the antibodies recognize extracellular or intracellular portions of each. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. CUT&Tag was performed with Hep G2 cells treated with Thapsigargin #12758 (300nM) for 4h and ATF-4 (D4B8) Rabbit mAb, using CUT&Tag Assay Kit #77552. The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3). The absence of signal in the HCT 116 knockout cells confirms the specificity of the antibody for LC3B. Western blot analysis of extracts from HeLa, C2C12, and KNRK cells, untreated (-) or treated with Chloroquine (50 μM, overnight; +) #14774 using LC3B (E5Q2K) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Cell signaling is a fundamental property of all cellular life in prokaryotes and eukaryotes . Typically, the signaling process involves three components: the signal, the receptor, and the effector. [citation needed] In biology, signals are mostly chemical in nature, but can also be physical cues such as pressure, voltage, temperature, or light.At Cell Signaling Technology (CST), our goal is to provide highly specific antibodies that yield strong, specific signal with minimal background. Our scientists screen a large number of antibodies and recommend only those best suited for the application. Our validation efforts include extensive protocol optimization and antibody titrationCiteAb’s 2022 analysis shows Cell Signaling Technology has maintained a leading position as the vendor with the most antibodies in the top 100. This figure now stands at 36, an …The absence of signal in the HCT 116 knockout cells confirms the specificity of the antibody for LC3B. Western blot analysis of extracts from HeLa, C2C12, and KNRK cells, untreated (-) or treated with Chloroquine (50 μM, overnight; +) #14774 using LC3B (E5Q2K) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Checking the Cell Signaling Technology ® (CST ®) website, you are in luck! There are mouse reactive flow-validated antibody conjugates available against each target: CD4 …Keratin 19 (K19) is expressed in glandular epithelia, including the liver, gallbladder, and pancreas, as well as in adenocarcinomas of the breast, thyroid, and bile duct. Keratin 20 (K20) is expressed in gastrointestinal epithelium, urothelium, and Merkel cells in the skin, as well as in colorectal carcinomas and some urothelial carcinomas.Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses, including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182.In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca 2+ /calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3).The Harper Lab studies mechanisms underlying cellular homeostasis and signaling, with a focus on the ubiquitin system and the autophagy-lysosome system. The interest in the …Cell Signaling Technology (CST) is a different kind of life sciences company—one founded, owned, and run by active research scientists, with the highest standards of product and …Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6,7).Lane 1 is 10% input, lane 2 is USP18 (D4E7) Rabbit mAb, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900. Western blot analysis was perfomed using USP18 (D4E7) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody. To Purchase # 4813. Cat.If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Directions for Use: Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBST. Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Western blot analysis was performed using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody. Confocal immunofluorescent analysis of HeLa cells, serum starved (left) or TNF-α treated (#8902 at 20 ng/ml for 20 min, right), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb ... Cell Signaling Technology (CST) | 21,261 followers on LinkedIn. CST is a private, family-owned company, founded by scientists and dedicated to providing high-quality research tools. | Founded by ...Poster: Signaling Pathways | Cell Signaling Technology. This poster provides a comprehensive overview of the major signaling pathways involved in cell growth, survival, differentiation, and stress responses. Download the PDF to explore the key molecules and interactions that regulate these pathways and their implications for human health and …The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. CUT&RUN was performed with 293 cells treated with Forskolin #3828 (30 μM) for 1h and Phospho-CREB (Ser133) (87G3) Rabbit mAb, using CUT&RUN Assay Kit #86652.Receptors are the main switches initiating downstream short-range and long-distance signaling in the cell and modulating cellular activities (Czyzewicz et al. …Ferroptosis is an iron-dependent form of regulated cell death associated with an increase in lipid peroxides (reviewed in 1,2). Free divalent iron (Fe2+) can lead to spontaneous lipid peroxidation through a Fenton reaction. Ferroptosis is regulated by signaling pathways that control iron storage and oxidative stress.1X Animal-Free Blocking Solution: to 4 mL of dH O add 1 ml of Animal-Free Blocking Solution (5X) ( #15019 ). Incubate sections in three washes of xylene for 5 minutes each. Incubate sections in two washes of 100% ethanol for 10 minutes each. Incubate sections in two washes of 95% ethanol for 10 minutes each.Western blot analysis of extracts from various cell lines using GAPDH (D16H11) XP ® Rabbit mAb. Simple Western™ analysis of lysates (0.1 mg/mL) from HeLa cells using GAPDH (D16H11) XP ® Rabbit mAb #5174. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody.Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows ...Apr 15, 2022 · Cell Signaling Technology (CST) emphasizes that it was founded by scientists, for scientists. This is at the core of the company’s culture and permeates all its decisions. Headed by neuroscientist Michael Comb, CST seeks to deeply understand the requirements of their customers—bench researchers who are in the weeds of complex research ... IHC-validated Antibodies for Cellular Senescence. Accelerate and increase confidence in your research results with IHC-validated p16 INK4A (BC42). An antibody shouldn’t be one of the variables in your experiment. Find out why customers …Cell Signaling Technology is a private, family-owned company that operates in the field of applied systems biology research, particularly focusing on cancer. The company's main offerings include the development and production of antibodies, which are validated for multiple applications by their team of Ph.D. level scientists. Learn & Support. Use this Directory to navigate to a wealth of informative resources about molecular targets and the protocols and tools needed to study them efficiently. Under "Education & Resources" you will find links to a large collection of educational resources, including our PhosphoSitePlus® PTM Database, useful reference tables, guides ... Lane 1 is 10% input, lane 2 is USP18 (D4E7) Rabbit mAb, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900. Western blot analysis was perfomed using USP18 (D4E7) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody. To Purchase # 4813. Cat.The Rb (4H1) Mouse mAb confirms silencing of Rb expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Rb siRNA. Western blot analysis of extracts from COS-7 cells, untreated or hydroxyurea-treated (G1/S), using Rb (4H1) Mouse mAb. Immunohistochemical analysis of paraffin-embedded human breast ... Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is β3-Tubulin (D71G9) XP ® Rabbit mAb. Western blot analysis was performed using β3-Tubulin (D71G9) XP ® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as the secondary antibody. Background. TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Cell-cell signaling involves the transmission of a signal from a sending cell to a receiving cell. However, not all sending and receiving cells are next-door neighbors, nor do all cell pairs exchange signals in the same way. There are four basic categories of chemical signaling found in multicellular organisms: paracrine signaling, autocrine ... NKX2.5 plays an essential role in heart development and is among the earliest factors expressed in the cardiac lineage in developing embryos. Targeted disruption of the murine Nkx2.5 gene results in abnormal heart morphogenesis, severe growth retardation, and embryonic lethality around E9.5 (1,2).PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197 and 1217 is correlated with PLCγ2 activity (9,10). Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509. Smrcka, A.V. et al. (1991) Science 251, 804-7.Actin filaments have been labeled with DY-554 phalloidin (red). Flow cytometric analysis of HeLa cells (blue) and NTERA-2 cells (green) using Nanog (D73G4) XP ® Rabbit mAb. Western blot analysis of extracts from NCCIT, NTERA-2 and iPS cells using Nanog (D73G4) XP ® Rabbit mAb. Immunohistochemical analysis of paraffin-embedded human …CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and … CiteAb’s 2022 analysis shows Cell Signaling Technology has maintained a leading position as the vendor with the most antibodies in the top 100. This figure now stands at 36, an increase of one over 2021, which is double that of the second most cited supplier. In addition, our antibodies now hold all three top spots, with our Anti-rabbit IgG ... If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation. Cell-cell signaling involves the transmission of a signal from a sending cell to a receiving cell. However, not all sending and receiving cells are next-door neighbors, nor do all cell pairs exchange signals in the same way. There are four basic categories of chemical signaling found in multicellular organisms: paracrine signaling, autocrine ... Polyclonal Antibody for studying Flag-Tag. Cited in 774 publications. Validated for WB, IP, F. Available in 2 sizes. Highly specific and rigorously validated in-house, DYKDDDDK Tag Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (CST #2368) is … Product Description. This Cell Signaling Technology antibody is tested in-house for indirect flow cytometric analysis in human cells and is expected to react with cell surface expressed CARs of varying specificity, which contain a G4S linker within the scFv of the extracellular domain. Product Description. The Senescence β-Galactosidase Staining Kit is designed to conveniently provide reagents needed to detect β-galactosidase activity at pH 6, a known characteristic of senescent cells. Papers have published using this kit in both cells and frozen tissue. The kit includes all reagents necessary for this assay.Western blot analysis of extracts from control HeLa cells (lane 1) or Vimentin knockout HeLa cells (lane 2) using Vimentin (D21H3) XP ® Rabbit mAb #5741 (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower). The absence of signal in the Vimentin knockout HeLa cells confirms specificity of the antibody for Vimentin. Show More.CUT&RUN was performed with 100,000, 10,000, or 5,000 HCT 116 cells (as indicated) and Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751, using the CUT&RUN Assay Kit. DNA Libraries were prepared using DNA Library Prep Kit for Illumina ® (ChIP-seq, CUT&RUN) #56795. This figure shows enrichment around the GAPDH gene, a known …Simple Western™ analysis of lysates (0.1 mg/mL) from Raji cells using Mcl-1 (D35A5) Rabbit mAb #5453. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 ... GPX4 converts lipid hydroperoxides to non-toxic lipid alcohols, therefore preventing ferroptosis (2). Research studies show that selenium enhances GPX4 expression and inhibits ferroptotic death to protect neurons (3). In addition, some therapy-resistant cancer cells depend on GPX4 to survive. Rab -Rabbit. All -All Species Expected. Polyclonal Antibody for studying H2AX (Ser139) phosphate. Cited in 1048 publications. Validated for WB, WB, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Histone H2A.X (Ser139) Antibody (CST #2577) is ready to ship.Specificity and sensitivity of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody. The antibody reacts specifically with as little as 0.25 ng of phosphorylated p42 MAP kinase and does not cross-react with up to 4 µg of nonphosphorylated p42 MAP kinase. Western blot analysis of whole-cell extracts from unstarved wild-type mouse embryonic ...Cell Signaling Technology (CST) is dedicated to providing the world’s highest quality, innovative research products to accelerate biological understanding. A privately …Species Cross-Reactivity Key: Polyclonal Antibody for studying eNOS. Cited in 167 publications. Validated for Western Blotting. Highly specific and rigorously validated in-house, eNOS Antibody (CST #9572) is ready to ship.Keratin 19 (K19) is expressed in glandular epithelia, including the liver, gallbladder, and pancreas, as well as in adenocarcinomas of the breast, thyroid, and bile duct. Keratin 20 (K20) is expressed in gastrointestinal epithelium, urothelium, and Merkel cells in the skin, as well as in colorectal carcinomas and some urothelial carcinomas.Lane 1 is 10% input, lane 2 is USP18 (D4E7) Rabbit mAb, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900. Western blot analysis was perfomed using USP18 (D4E7) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody. To Purchase # 4813. Cat.Apr 15, 2022 · Cell Signaling Technology (CST) emphasizes that it was founded by scientists, for scientists. This is at the core of the company’s culture and permeates all its decisions. Headed by neuroscientist Michael Comb, CST seeks to deeply understand the requirements of their customers—bench researchers who are in the weeds of complex research ... Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: H-Human M-Mouse R-Rat Hm-Hamster Mk-Monkey Vir-Virus Mi-Mink C-Chicken Dm-D. melanogaster X-Xenopus Z-Zebrafish B-Bovine Dg-Dog Pg-Pig Sc-S. …Western blot analysis was performed using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody. Confocal immunofluorescent analysis of HeLa cells, serum starved (left) or TNF-α treated (#8902 at 20 ng/ml for 20 min, right), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb ... Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film. Keratin 19 (K19) is expressed in glandular epithelia, including the liver, gallbladder, and pancreas, as well as in adenocarcinomas of the breast, thyroid, and bile duct. Keratin 20 (K20) is expressed in gastrointestinal epithelium, urothelium, and Merkel cells in the skin, as well as in colorectal carcinomas and some urothelial carcinomas.Immunoprecipitation of TIM-3 from RPMI 8226 cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is TIM-3 (D5D5R ™) XP ® Rabbit mAb, #45208. Western blot was performed using TIM-3 (D5D5R ™) XP ® Rabbit mAb. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4). Protein extracts from 3T3-Src cells were profiled by PhosphoScan ® to identify phosphotyrosine peptides. Tyr458 of PI3K p85 and Tyr199 of PI3K p55 were among 180 phosphopeptides and 185 phosphotyrosine sites identified (5). Cantley, L.C. (2002) Science 296, 1655-7. Cell-cell signaling involves the transmission of a signal from a sending cell to a receiving cell. However, not all sending and receiving cells are next-door neighbors, nor do all cell pairs exchange signals in the same way. There are four basic categories of chemical signaling found in multicellular organisms: paracrine signaling, autocrine ... The interactive pathways and diagrams have been created by CST scientists and outside experts to provide concise overviews of selected signaling pathways and cellular mechanisms. Protein nodes in each interactive diagram are linked to specific antibody product information, as well as protein-specific listings in the PhosphoSitePlus ® …Specificity / Sensitivity. Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full-length caspase-3 or other cleaved caspases.Monoclonal Antibody for studying H3 (Ser10) phosphate. Cited in 372 publications. Validated for WB, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit Monoclonal Antibody (CST #3377) is …Storage. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 31235.Cell Signaling Technology (CST) | 21,261 followers on LinkedIn. CST is a private, family-owned company, founded by scientists and dedicated to providing high-quality research tools. | Founded by ...Monoclonal Antibody for studying ACTA2 (alpha actin, smooth muscle). Cited in 560 publications. Validated for WB, WB, IP, IHC, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, α-Smooth Muscle Actin (D4K9N) XP® Rabbit Monoclonal Antibody (CST #19245) is ready to ship. Specificity / Sensitivity. Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins. DIVERSITY, EQUITY, & INCLUSION. CST understands the importance of a safe and diverse workplace. Different perspectives and ideas drive collaboration and innovation. By providing an inclusive and stimulating work environment, we aim to establish human dignity and meaningful work as an essential part of our culture. Embrace the. culture.At Cell Signaling Technology (CST), we understand that there is no single assay that can determine the validity of an antibody.Confirming that an immunoreagent is sufficiently specific and sensitive depends on the application and protocol being used, the type and quality of sample being analyzed, and the inherent biophysical properties of the antibody itself.Sandusky taxi, Kohls gastonia, Snoppy, Mo's deli aventura, Baylor women's, Nj com sports, Decorative rocks near me, Northstar theater, The plush horse, Hops burger bar, Whole foods woodbury, Uaa alaska, Golden arrow resort, Trees and trends
If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.. Lowe's dubois pa
In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca 2+ /calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3). Background. Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation ... The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is ...Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase.Storage. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 31235.Cell Signaling Technology (CST) is a different kind of life sciences company—one founded, owned, and run by active research scientists, with the highest standards of product and service quality ...Phospho-Akt substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery. Montminy, M. (1997) Annu Rev Biochem 66, 807-22. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, …Ferroptosis is an iron-dependent form of regulated cell death associated with an increase in lipid peroxides (reviewed in 1,2). Free divalent iron (Fe2+) can lead to spontaneous lipid peroxidation through a Fenton reaction. Ferroptosis is regulated by signaling pathways that control iron storage and oxidative stress. Specificity / Sensitivity. Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins. Cell-cell signaling involves the transmission of a signal from a sending cell to a receiving cell. However, not all sending and receiving cells are next-door neighbors, nor do all cell pairs exchange signals in the same way. There are four basic categories of chemical signaling found in multicellular organisms: paracrine signaling, autocrine ...Cyclin D is degraded through the ubiquitin proteasome pathway in the absence of mitogenic signaling. Ubiquitination of cyclin D1 is enhanced by phosphorylation at Thr286 by glycogen synthase kinase 3b (GSK-3b) (1). p27/Kip1, p57 Kip2 and p21 Waf1/Cip1 are members of the Cip/Kip family of cyclin-dependent kinase inhibitors.Background. TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4).Immunoprecipitation of ATF-6 from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is ATF-6 (D4Z8V) Rabbit mAb. Western blot analysis was performed using ATF-6 (D4Z8V) Rabbit mAb. Western blot analysis of extracts from 293T cells, untreated (-) or treated with tunicamycin (1 mM ...Cell Signaling Technology (CST) is a different kind of life sciences company—one founded, owned, and run by active research scientists, with the highest standards of product and …Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses, including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182.Monoclonal Antibody for studying ACTA2 (alpha actin, smooth muscle). Cited in 560 publications. Validated for WB, WB, IP, IHC, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, α-Smooth Muscle Actin (D4K9N) XP® Rabbit Monoclonal Antibody (CST #19245) is ready to ship. Background. Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation ... Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. Cathepsin B (D1C7Y) XP ® Rabbit mAb recognizes endogenous levels of total cathepsin B protein.Species Cross-Reactivity Key: Polyclonal Antibody for studying eNOS. Cited in 167 publications. Validated for Western Blotting. Highly specific and rigorously validated in-house, eNOS Antibody (CST #9572) is ready to ship.Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: H-Human M-Mouse R-Rat Hm-Hamster Mk-Monkey Vir-Virus Mi-Mink C-Chicken Dm-D. melanogaster X-Xenopus Z-Zebrafish B-Bovine Dg-Dog Pg-Pig Sc-S. cerevisiae Ce-C. …Simple Western™ analysis of lysates (0.1 mg/mL) from COS-7 cells, transfected with a construct expressing HA-tagged Stat3 using HA-Tag (6E2) Mouse mAb #2367. The virtual lane view (left) shows a single target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody.Cell signaling is a fundamental property of all cellular life in prokaryotes and eukaryotes . Typically, the signaling process involves three components: the signal, the receptor, and the effector. [citation needed] In biology, signals are mostly chemical in nature, but can also be physical cues such as pressure, voltage, temperature, or light.Monoclonal Antibody for studying H3 (Ser10) phosphate. Cited in 372 publications. Validated for WB, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit Monoclonal Antibody (CST #3377) is …If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197 and 1217 is correlated with PLCγ2 activity (9,10). Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509. Smrcka, A.V. et al. (1991) Science 251, 804-7.IHC-validated Antibodies for Cellular Senescence. Accelerate and increase confidence in your research results with IHC-validated p16 INK4A (BC42). An antibody shouldn’t be one of the variables in your experiment. Find out why customers … Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4). Protein extracts from 3T3-Src cells were profiled by PhosphoScan ® to identify phosphotyrosine peptides. Tyr458 of PI3K p85 and Tyr199 of PI3K p55 were among 180 phosphopeptides and 185 phosphotyrosine sites identified (5). Cantley, L.C. (2002) Science 296, 1655-7. Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: Checking the Cell Signaling Technology ® (CST ®) website, you are in luck! There are mouse reactive flow-validated antibody conjugates available against each target: CD4 … Learn & Support. Use this Directory to navigate to a wealth of informative resources about molecular targets and the protocols and tools needed to study them efficiently. Under "Education & Resources" you will find links to a large collection of educational resources, including our PhosphoSitePlus® PTM Database, useful reference tables, guides ... Western blot analysis of extracts from various cell lines using GAPDH (D16H11) XP ® Rabbit mAb. Simple Western™ analysis of lysates (0.1 mg/mL) from HeLa cells using GAPDH (D16H11) XP ® Rabbit mAb #5174. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody.Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.Dana Seehale joined Cell Signaling Technology in February 2017 as Director, Lab Informatics, responsible for multiple technologies supporting Product Development and Production, including PDP/PLM, Core LIMS, and Jira. Her responsibilities were later expanded to include the PIM/STEP system and the Oracle EBS/EPortal platforms. Dana is now ...1X Animal-Free Blocking Solution: to 4 mL of dH O add 1 ml of Animal-Free Blocking Solution (5X) ( #15019 ). Incubate sections in three washes of xylene for 5 minutes each. Incubate sections in two washes of 100% ethanol for 10 minutes each. Incubate sections in two washes of 95% ethanol for 10 minutes each.Contact Technical Support. Hours: 8:00 AM - 6:00 PM (EST) Phone: 877-678-8324. Email: [email protected] 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.Cell Signaling Technology is a private, family-owned company that operates in the field of applied systems biology research, particularly focusing on cancer. The company's main offerings include the development and production of antibodies, which are validated for multiple applications by their team of Ph.D. level scientists.p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1,2). p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs (1). A second isoform, p85 S6 kinase, is derived from the same gene ...Monoclonal Antibody for studying LC3B. Cited in 138 publications. Validated for WB, IP, IHC, IF, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, LC3B (E5Q2K) Mouse Monoclonal Antibody (CST #83506) is ready to ship.Specificity / Sensitivity. Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full-length caspase-3 or other cleaved caspases.The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3).If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Monoclonal Antibody for studying H3 (Ser10) phosphate. Cited in 372 publications. Validated for WB, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit Monoclonal Antibody (CST #3377) is …If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6,7).The absence of signal in the HCT 116 knockout cells confirms the specificity of the antibody for LC3B. Western blot analysis of extracts from HeLa, C2C12, and KNRK cells, untreated (-) or treated with Chloroquine (50 μM, overnight; +) #14774 using LC3B (E5Q2K) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Apr 15, 2022 · Cell Signaling Technology (CST) emphasizes that it was founded by scientists, for scientists. This is at the core of the company’s culture and permeates all its decisions. Headed by neuroscientist Michael Comb, CST seeks to deeply understand the requirements of their customers—bench researchers who are in the weeds of complex research ... Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 #9903 (50 μM, 1 μM, and 10 μM, 2 hr; blue) using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (dashed lines). Anti-rabbit IgG …. 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